Development of Arabidopsis Protein Chip
Plant TAP Expression Vector

(attB1-LIC-F-CcdB-LIC-R-attB2-9xMYC-(HIS)6-3C site-2xIgG binding domain)

Features of the vector (11429 nucleotides)
35S promoter: 7799-8567
TMV-omega: 8568-8642
attB1: 8643-8667
CcdB: 8964-9269
attB2: 9320-9345
9xMYC: 9379-9732
(HIS)6: 9790-9807
3C cleavage site: 9808-9831
2xIgG binding domain: 9871-10245

Bacterial selection: spectinomycin

Plant selection: gentamycin

Background vector: pZP222 (the binary vector with the gentamycin marker) is U10463 the reference for the vector is Hajdukiewicz et al. 1994, Plant Mol. Biol. 25, 989-994. [Abtract]

Sequence: EU040203


Popescu SC, Popescu GV, Bachan S, Zhang Z, Seay M, Gerstein M, Snyder M, Dinesh-Kumar SP. (2007) Differential binding of calmodulin-related proteins to their targets revealed through high-density Arabidopsis protein microarrays. Proc. Natl. Acad. Sci. USA 104:4730-4735. [Abstract]